Polymorphism Evaluation of TLR2 Gene Associated with Endometritis Infection in Buffalo Reared in Egypt

Background and Aim: Toll-like receptors (TLRs) are important for the recognition of pathogen-associated molecular patterns. Single nucleotide polymorphisms (SNPs) within TLRs have a potential impact on alteration susceptibility or resistance to inflammatory diseases. This work focused case-control study distribution SNPs in TLR2 gene test their role endometritis occurrence river buffalo.
 Materials Methods: Egyptian buffaloes from slaughterhouse were tested, where forty samples divided into apparently healthy uteri (20n) clinically infected ones used detect genetic association between disease.
 Results: Nineteen novel polymorphic sites identified. Three found be statistically significant. AA genotype at 5`UTR CC coding region had significant with endometritis, while GG endometritis. Polyphen 2 analysis revealed three amino acids substitutions protein having functional significance. Haplotype reconstruction statistical significance haplotype frequencies cases controls indicating its endometritis.
 Conclusions: It is concluded that innate immune activation response may interfered by effect mutations due reduction ability recognize Pathogen associated Molecular pattern (PAMPs).